Instrumental Analysis

Biol 320

Syllabus------Fall 2007

Time: Lecture : Mon., Fri.,  2:00 p.m. to 3:15 p.m.
Location:  Lecture: Capwell 114, Lab: Capwell 102
Professor: Joseph Falcone Ph.D
Office: 305 Capwell Hall 
Phone: 570-945-8413
Office Hours: Mon-Fri 9:00a.m. and by appointment. 

Text:  The text for this course is Principles of Instrumental Analysis, 6th ed. ,  Skoog,  Holler, and Crouch

Prerequisite:  Chem 105-106 and/or permission of instructor.

Required:  Scientific calculator, lab notebook.


COURSE DESCRIPTION: This course is designed to give the student a broad experience in the theory of modern laboratory instrumentation. The labs will be investigative in nature where students are required to use the Internet and the current literature to explore practical ways of using instruments for solving chemical, biological, environmental problems qualitatively and quantitively. The students will survey the theory and application of instruments such as: visible, ultraviolet, Fluorescence, Nuclear Magnetic Resonance, Atomic Emission, Chromatography, and Mass Spectrometry.

COURSE OBJECTIVES: Upon successful completion of this course, the student should be able to:

1. understand and appreciate the role of instruments in solving problems in the physical, chemical and biological sources.
2. understand the theoretical concepts behind each instrument.
3. operate, manipulate, and generate data for each instrument.
4. appreciate the connectivity between math, physics, chemistry, and biology in this course.
5. solve problems quantitatively and qualitatively by making appropriate choices among the various instruments.
6. appreciate the complexity of each instrument, its strength, and its limitation.
7. explore the use of Internet and Library as an educational source in instrumentation


1.Attendance is required.
2. Homework problems will be assigned and graded.
3. A complete portfolio of all homework, experiments, lab exercises, data, and lab reports.
4. A research project.
5. A comprehensive final examination
6. Academic integrity must be always preserved. Any violation will be handled according to the guidelines of the College Catalog.

LAB REPORTS: You may design your reports in any way you wish, as long as reports and graphs are not hand written, and contains the following information:

1.      your name, title of each exercise, date, and a statement of objective;

2.      all generated data, spectra, and graphs;

3.      a discussion of the data generated from lab exercises;

4.      a discussion of and conclusion to your unknowns' identity in a neat and orderly fashion; and

5.      a brief discussion and conclusion for the project.

Set up a conference with me ASAP to discuss areas of interest for possible projects.  Upon selection of a project, the student will become familiar with current literature regarding the science and instrumentation required for the chosen topic.  The student will acquire data on selected instruments and prepare a poster and/or powerpoint presentation upon completion of the chosen project.     


The following instruments are available:

            A Cary 50 UV-Visible spectrophotometer

            A Cary Fluorescence spectrophotometer

            A Varian  High Performance Liquid Chomatograph

            A Varian 1200L GC/LC Mass Spectrometer

            A Varian ICP Optical Emission Spectrometer



             Homework/Exams                               30%
             Experiments                                         30%
             Final Exam                                          40%
             Total                                                   100%

Bonus Research Project

Letter grades translate as follows:
A 95-100%, A- 90-94.9% B+ 86-89.9%, B 80-85.9%
B- 77-79.9% C+ 75-76.9% C 68-74.9% C- 64-67.9%
D 60-63.9% F <60% or as a consequence of academic dishonesty.
I in extreme circumstances and with permission of professor.

         Keystone College does not discriminate in any of its programs on the basis of disability.  While there is not a deadline for the disclosure of a disability by a student, in order to facilitate the documentation and accommodation processes, students are encouraged to voluntarily and confidentially disclose any disability requiring an accommodation prior to the beginning of class.  This disclosure should be made to Robert Iannuzzo, Vice President of Enrollment, Keystone’s Section 504/ADA